Ha Thi Hue
Poster Presenter
Hue Aromatic Betel Bokashi for Controlling White Faeces Syndrome in EHP-Infected (Litopenaeus vannamei): Preliminary Results and Economic Prospects: White Faeces Syndrome (WFS) in (Litopenaeus vannamei) whiteleg shrimp is a multifactorial condition that may be associated with (Enterocytozoon hepatopenaei) (EHP) infection, gut microbiota dysbiosis, and opportunistic pathogens. This field study reports preliminary observations on using Hue betel leaf Bokashi—a preparation made from betel leaves combined with Lactobacillus spp. (L. casei) and (L. plantarum)—to assist in controlling WFS in ponds where EHP was detected. The team mixed the preparation into pelleted feed at a 2% inclusion rate for 5–7 days, using squid oil as a binder. Initial testing confirmed that all nine samples were positive for EHP. Preliminary verbal reports from the testing facility indicated that, following the intervention, the majority of follow-up tests were negative, with 10% remaining positive; formal re-test reports and comparative sample data are pending confirmation. Consequently, it is not possible to attribute the shift to negative status specifically to betel leaf Bokashi or to determine the treatment success rate. Regarding the mechanism of action, eugenol and phenolic compounds in betel leaves maay have potential antimicrobial properties, while *Lactobacillus* spp. may help modulate gut microbiota. However, the synergistic effects and the potential for direct action against EHP remain to be demonstrated. At a price of 150,000 VND/L, the cost of the preparation is estimated at approximately 3,000 VND per kilogram of feed when used at a dosage of 20 mL/kg; this figure excludes the cost of squid oil and labour. Further research incorporating a control group, qPCR for EHP quantification, and assessments of WFS expression, FCR, survival rate, and profitability is required to determine biological and economic efficacy. Keywords: Betel leaf Bokashi; Eugenol; Lactobacillus spp.; EHP; White Faeces Syndrome; economic efficiency.