Alireza Dehnad
Biography
Green Synthesis of Gold Nanoparticles by Microbial. Extensive research on viral diseases in birds and humans (Pox, Newcastle, Influenza). Extensive research on bacterial diseases in Domestic animals and humans (Staphylococcus aureus, Burkholderia). Application of CRISPR / Cas9 system . Identification of CRISPR / Cas9 system in bacteria. Optimization of antibiotic production processes, Isolation and identification of antibiotic producing Actinomycetes, Bio corrosion, Cell immobilization technique. Comparative Biochemistry and Kinetics of industrial enzyme made by microbial fermentation. Molecular, chemical and biological screening of soil Actinomycete isolates in seeking bioactive peptide metabolites. Systematic and biotechnological aspects of hemophilic and halo tolerant Actinomycetes. Metabolomics study on colon cancer , immunomodulatory and ……. Isolation of microbial natural products (extraction of secondary metabolites from Actinomycete). Biotechnological application and taxonomical distribution of plant growth promoting Actinobacteria. Isolation, identification, evaluation of the ability and production of probiotic bacteria in bioreactor. Identification of bacteria with probiotic properties and investigation of post-biotic effects. Production and extraction by fermentation.... Production of L - asparaginase, an anticancer agent and selenium supplement. Developments in Microbial Biotechnology and Bioengineering , Bioremediation and Biodegradation. Optimization isolation of biopolymer producing bacteria using as controlled release drug delivery. Research on wound healing using nanofibers. Secondary metabolites of native isolates the treatment of infectious diseases and wound healing in mice. Experimental Design by Taguchi Method. Fermentation Microbiology and Biotechnology, Screening of Industrial Microorganisms. Determination of the prevalence in Iranian apiaries.
Research Interest
Assistant Professor of Microbiology , Department of Diseases Research, Deputy Directorship for Research and Technology,Razi Vaccine and Serum Research Institute , AREEO, Tabriz- IRAN
Abstract
The Examination Ff Some Virulence Factors In S. Aureus Isolates Obtained From The Healthy Human Population, Sheep Mastitis,And Cheese
Background: Staphylococcus aureus is responsible for many infections in humans and animals from skin and soft tissue infections to life-threatening diseases. In this study to explore the origin of S. aureus infections in humans, the antibiotic resistance profile and he variety of virulence factors in S. aureus isolates were examined in three groups: a healthy human population, cheese, and the milk of sheep with mastitis. Aims: The examination of some virulence factors in S. aureus isolates obtained from the healthy human population, sheep mastitis, and cheese. Methods: A total of 400 nasal swab samples from healthy students, 30 cheese samples, and 122 sheep milk samples were collected for the detection of S. aureus isolates from January 1, 2018, to March 1, 2018. The frequency of hla, hlb, Acme/arcA, pvl, and tsst-1 virulence genes and mecA gene was determined in each group by PCR assay. Results: There was a direct relationship between the antibiotic susceptibility profile of the isolates from a healthy population and those from mastitis milk samples. Of 400 nasal samples, 15% (60/400) were positive for S. aureus, of which 60% (36/60) were positive for mecA. While 50% (15/30) of cheese samples were positive for S. aureus. of which 7 cases (46.66%, 7/15) were positive for mecA. The prevalence of S. aureus among students was dependent on gender (P=0.025). Also, 47.5% (58/122) of milk samples from sheep mastitis were positive for S. aureus, and 41.37% (24/58) were positive for the mecA gene. Based on PCR results, the highest rate of hla (68.33%, 41/60), hlb (53.33%, 32/60), and Acme/arcA (46.66%, 28/60) genes were related to a healthy population, and the highest frequencyof pvl (41.38%, 24/58), and tsst-1 (27.59%, 16/58) was related to milk samples (P<0.05). A significant correlation was observed between the presence of the arginine catabolic mobile element (ACME)-arcA gene and resistance to methicillin (P<0.05). Conclusion: The high rate of virulence factors in the S. aureus isolates obtained from mastitis and dairy products is an alert point, because they could be source of the spreading of S. aureus to humans. There is an essential need for continuous monitoring to control staphylococcal food poisoning.
Keywords: Dairy products, Healthy population, Mastitis, S. aureus, Virulence factors
